Reporter
Part:BBa_K1100018:Design
Designed by: Chenxi He, Jiadong Ni Group: iGEM13_Fudan (2013-09-13)
R1051 with insulator and sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To test the stochastic dynamics of the constitutive promoters by means of measuring fluorescence intensity of sfGFP using flow cytometry.
Source
R1051 from Kit, Insulator: Csy4 loci via PCR, sfGFP from Ding Yu lab at Fudan University
References
Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.